The objective of this experiment was to determine the effects of providing an increased amount of milk on periprandial glucagon-like peptide-2 (GLP-2) concentrations in pre-weaned dairy calves. Nineteen female Holstein calves were randomly assigned to 1 of 2 treatments on d 2 after birth; HIGH (10 L/d; n = 9) or LOW (5 L/d; n = 10) amount of pasteurized whole milk. All calves were allowed 2.5 L of milk per meal until d 50 before a 10-d weaning transition began. Calves were housed in individual pens for the first 21 ± 3 d, before being moved to a group pen and fed using an automated calf feeder. Calf starter was provided ad libitum from d 21 ± 3 d. At wk 3 (before being moved to the group pen), wk 5, and wk 7 (before the weaning transition) of life, a series of blood samples were collected relative to their morning milk meal, which was at least 6 h after a previous milk meal. Overall, HIGH calves tended to have increased mean plasma GLP-2 concentrations on wk 3 (P = 0.10) and wk 5 (P = 0.08), but no difference was observed on wk 7. Similarly, pre-meal concentration of plasma GLP-2 tended to be greater for HIGH calves compared with LOW at wk 3 (1.05 vs. 0.77 ng/mL; P = 0.11), and was greater at wk 5 (0.76 vs. 0.44 ng/mL; P = 0.04), however no difference was observed at wk 7 (0.56 vs. 0.47 ng/mL; P = 0.77). Although starter intake of HIGH calves tended to be lower than LOW calves (241 vs. 413 g/d; P = 0.06) for wk 4 to 7, there was a negative correlation between starter intake and pre-meal GLP-2 concentrations (r = 0.30; P = 0.02) at wk 5, indicating that plasma GLP-2 concentration of pre-weaned dairy calves can be increased to a greater extent by milk intake than starter intake. Plasma glucose concentrations were not different among treatments at any measured time point; however plasma insulin concentration tended to be higher for HIGH calves on wk 3 (P = 0.08). These results suggest that feeding more milk early in life can increase plasma GLP-2 concentrations in pre-weaned dairy calves.