Abstract #210
Section: Ruminant Nutrition (orals)
Session: Ruminant Nutrition II: Methane
Format: Oral
Day/Time: Monday 2:30 PM–2:45 PM
Location: Ballroom G
Session: Ruminant Nutrition II: Methane
Format: Oral
Day/Time: Monday 2:30 PM–2:45 PM
Location: Ballroom G
# 210
Dose-response effect of 3-nitrooxypropanol on enteric methane emission in dairy cows.
A. Melgar*1, K. C. Welter2, K. Nedelkov3, C. M. M. R. Martins2, M. T. Harper1, J. Oh1, S. E. Räisänen1, X. Chen4, S. F. Cueva5, S. Duval6, A. N. Hristov1, 1The Pennsylvania State University, University Park, PA, 2University of Sao Paulo, Pirassununga, Brazil, 3Faculty of Veterinary Medicine, Trakia University, Stara Zagora, Bulgaria, 4College of Pastoral Agriculture Science and Technology, Lanzhou University, China, 5Panamerican Agricultural University, Zamorano, Francisco Morazán, Honduras, 6Research Centre for Animal Nutrition and Health, DSM Nutritional Products, France.
Key Words: 3-nitrooxypropanol, enteric methane, dairy cattle
Dose-response effect of 3-nitrooxypropanol on enteric methane emission in dairy cows.
A. Melgar*1, K. C. Welter2, K. Nedelkov3, C. M. M. R. Martins2, M. T. Harper1, J. Oh1, S. E. Räisänen1, X. Chen4, S. F. Cueva5, S. Duval6, A. N. Hristov1, 1The Pennsylvania State University, University Park, PA, 2University of Sao Paulo, Pirassununga, Brazil, 3Faculty of Veterinary Medicine, Trakia University, Stara Zagora, Bulgaria, 4College of Pastoral Agriculture Science and Technology, Lanzhou University, China, 5Panamerican Agricultural University, Zamorano, Francisco Morazán, Honduras, 6Research Centre for Animal Nutrition and Health, DSM Nutritional Products, France.
This experiment was designed to test the effect of inclusion rate of 3-nitrooxypropanol (3NOP), a methane inhibitor, on enteric methane emission in dairy cows. The study was conducted with 49 multiparous cows (155 ± 7.7 DIM, 634 ± 8.1 kg BW, and 44 ± 1.1 kg/d milk yield) in a randomized complete block design. The experiment was conducted in 2 phases; phase 1 was with 21 cows and phase 2, with 28 cows. Cows were blocked based on DIM, milk yield, and enteric methane emission during a 14-d covariate period. Cows were fed ad libitum a basal TMR diet containing (%, DM basis): corn silage, 50; alfalfa haylage, 10; and concentrate feeds and mineral-vitamin premix, 40. Treatments were control (placebo, no 3NOP) and 40, 60, 80, 100, 150, and 200 mg 3NOP/kg feed DM. Following a 14-d adaptation period, enteric gaseous emissions (methane, carbon dioxide, and hydrogen) were measured in 3 d using the GreenFeed system (C-Lock Inc., Rapid City, SD) at 0900, 1500, and 2100 h (d 1), 0300, 1200, and 1700 h (d 2), and 0000 and 0500 h (d 3). Dry matter intake (24.6 kg/d; SEM = 1.12), milk yield (44.0 kg/d; SEM = 1.15), and cow BW (634 kg; SEM = 5.2) were not affected (P ≥ 0.33) by treatment. 3NOP linearly decreased (P < 0.001) methane emission by an average of 31% (303 vs. 441 g/cow/d for the control; SEM = 25.7). The 3 highest 3NOP inclusion rates had a similar methane mitigation effect (average of 267 g/d, or a 40% reduction; P ≥ 0.93). 3NOP linearly decreased (P < 0.001) methane yield (average of 12.6 vs. 17.0 g/kg DMI for the control; SEM = 1.15) and methane emission intensity (7.1 vs. 9.7 g/kg milk, respectively; SEM = 0.63). Emission of hydrogen linearly increased (P < 0.001) from 0.3 g/cow/d for the control to an average of 2.2 g/cow/d (SEM = 0.42) for the 3 highest 3NOP inclusion rates. Emission of carbon dioxide was not affected by treatment (average of 13.7 kg/cow/d; SEM = 6.16; P = 0.97). In this experiment, 3NOP linearly decreased enteric methane production in dairy cows. Maximum mitigation effect was achieved at 100 mg/kg feed DM with no further decrease in enteric methane emission with 150 and 200 mg/kg 3NOP.
Key Words: 3-nitrooxypropanol, enteric methane, dairy cattle
