The objective of this study was to determine the effects of a novel RUP source (ADM) on nutrient digestion and performance by Holstein cows. A total of 18 Holstein cows [BW (653.0 ± 96.5 kg); DIM (123 ± 50 d)] were assigned to 1 of 3 treatments in a replicated 3 × 3 Latin Square Design with experimental periods 28 d in length. Periods were divided into an adaptation phase (d 1 to 21) and measurement phase (d 22 to 28). Treatments were CON, cows were fed a control diet (16% CP, 70% RDP, and 30% RUP); AMP, cows fed a diet with RUP source 1 (16% CP, 60% RDP, and 40% RUP); and ADM, cows fed a diet with RUP source 2 (16% CP, 60% RDP, and 40% RUP). Fecal grab samples were collected on d 25 to 27 of each period so that every 3 h in a 24-h period were represented (n = 8). Total mixed rations, orts, and fecal samples were composited by cow within each period and analyzed for DM, OM, CP, aNDF. Indigestible NDF was used as an internal marker. Spot urine samples were obtained approximately 6 h pre-feeding and 6 h post-feeding on d 20 of each period. Daily urinary volume and excretion of urea N, total N, and allantoin were estimated from urinary creatinine concentration assuming a creatinine excretion rate of 29 mg/kg of BW. Data were analyzed using the MIXED procedure of SAS. Contrast 1 (CONT1): CON compared with ADM and Contrast 2 (CONT2): AMP compared with ADM. Nitrogen intake and milk protein N was greater for cows in CON (639.4 ± 24.1 and 178.5 ± 5.0 g/d, respectively) than cows in AMP (576.2 ± 24.1 and 168.5 ± 5.0 g/d, respectively P = 0.01, CONT1). However, milk protein N as a percentage of N intake tended to be greater for cows in AMP than cows in CON (30.05 and 28.50 ± 1.12%, respectively, P = 0.07, CONT1). Dry matter intake was greater for cows in CON than cows in AMP (22.51 and 20.31 ± 0.90 kg/d, respectively, P = 0.01, CONT1). Crude protein intake was greater for cows in CON than cows in ADM (3.99 and 3.55 ± 0.15 kg/d, respectively, P = 0.01, CONT1). There were no treatment differences for CONT2 for DMI, N intake, protein N milk protein N as a proportion of N intake, DMI, or CP intake (P > 0.10). In conclusion, cows fed ADM novel RUP exhibited better N utilization than CON, thereby reducing environmental impact.