The objective was to evaluate the effect of supplementing Ca-salts of fish oil or Ca-salts of soybean oil during the last 3 wk of gestation and during the preweaning period of calves on growth performance of Holstein calves. During the last 3 wk of pregnancy, Holstein cattle (n = 120) were fed no fat supplement (CON), supplemented with Ca-salts of soybean oil (CSO, 140 g/cow/daily) or supplemented with Ca-salts of fish oil (CFO, 140 g/cow/daily). Eighty-four Holstein calves were used (n = 14 calves per treatment) in a completely randomized design, with dietary treatments in a 3 × 2 factorial arrangement (3 dam diets and 2 calf starters; n = 6). Newborn calves were fed a starter diet with either no fat supplement (FC-0) or supplemented with 2% Fat Ca-Salt (FC-2; contained 85% fat (1% C14:0, 28% C16:0, 3% C16:1, 5% C18:0, 26% C18:1, 30% C18:2, 3% C18:3, 4% others) and 9% Ca produced by Persiafat, Kimiya Danesh Alvand Co., Tehran, Iran). Total intake of starter during all the experimental period was not affected by type of calf starter (CS) or dam diets. Calves born from cattle fed CFO and CSO prepartum tended (P= 0.07) to have greater overall ADG compared with calves born from cattle fed no fat supplement prepartum (648, 643 versus 609 g/d, respectively). Calves fed the FC-2 CS had consistently greater ADG and feed efficiency (FE; kg of BW gain/kg of DMI) compared with calves fed the FC-0 CS (640 and 0.537 vs. 580 and 0.469, respectively). Calves born from dams fed fat tended (P = 0.09) to have greater weaning weight (WW) than those born from dams fed CON, but calves fed FC-2 CS had greater WW and final weight during the experimental period. Result indicated that feeding moderate amounts of polyunsaturated long-chain fatty acids during the last weeks of uterine life and preweaning time could improve ADG and FE on newborn calf.