We evaluated the effects of altering the ratio of palmitic (C16:0; PA) and oleic (cis-9 C18:1; OA) acids in supplemental fat on total-tract digestibility responses of dairy cows. Cows were blocked by milk yield and assigned to 3 groups (12 cows per group) in a main plot. Production groups were: a) low (45.2 ± 1.7 kg/d); b) medium (53.0 ± 1.6 kg/d); and c) high (60.0 ± 1.9 kg/d). Within each production group, a truncated Latin square arrangement of FA treatments was used in 2 consecutive 35-d periods with the final 5-d used for sample and data collection. The fatty acid (FA) treatments supplemented at 1.5% diet DM were: 1) 80:10 (80% PA + 10% OA); 2) 73:17 (73% PA + 17% OA 3) 66:24 (66% PA + 24% OA); and 4) 60:30 (60% PA + 30% OA). The statistical model included the random effect of cow within production group, and the fixed effect of treatment, production group, period, and their interactions. Linear, quadratic, and cubic contrasts were used to determine effects of increasing dietary OA. Results are presented in the following sequence: 80:10, 73:17, 66:24, and 60:30. We observed no treatment by production group interactions for any variable of interest. FA treatments did not affect intakes of DM (29.1, 29.0, 28.9 and 29.3 kg/d; P = 0.68), NDF (8.43, 8.42, 8.40, and 8.35; P = 0.66), or total FA (1164, 1148, 1132, and 1142 g/d; P = 0.70). Increasing OA in FA treatments decreased the intake of 16-carbon FA (465, 425, 379, and 370 g/d; P < 0.01, linear) and increased the intake of 18-carbon FA (644, 661, 693, and 721 g/d; P < 0.01, linear). Increasing OA in FA treatments increased total FA digestibility (76.5, 80.1, 79.9 and 80.6%; P = 0.02, linear), 16-carbon FA digestibility (72.9, 76.8, 75.2 and 78.1%; P < 0.01, linear), and 18-carbon FA digestibility (79.8, 83.5, 82.8 and 82.7%; P = 0.05, quadratic). Increasing OA in FA treatments decreased absorbed 16-carbon FA (337, 326, 285 and 287 g/d; P = 0.03, quadratic), increased absorbed 18-carbon FA (518, 551, 573 and 595 g/d; P < 0.01, linear), and tended to increase absorbed total FA (896, 921, 900, 924 g/d; P = 0.1, quadratic). Our results demonstrate that increasing OA up to 30% in a FA supplement increased FA digestibility and did not affect nutrient intake.