The objective was to examine the effect of methionine supply during the periparturient period on peripheral insulin sensitivity via glucose tolerance test and glucose utilization by skeletal muscle in dairy cows. Multiparous cows were randomly assigned to a control diet or the control plus rumen-protected methionine (MET; Mepron, Evonik Nutrition & Care GmbH). Mepron was fed from −28 to 30 d relative to parturition at a rate of 0.09% and 0.10% of DMI during the prepartum and postpartum period, respectively, to ensure a ratio 2.8:1 Lys to Met in the metabolizable protein. The glucose tolerance test was conducted at day −12 and +12 relative to calving in 12 cows per treatment. A sterile solution of 50% dextrose was administered intravenously at a dose of 0.25 g of glucose per kg of BW over the course of 5 min. Blood samples were collected from the tail vein or artery at −15, −5, 5, 10, 15, 30, 60, 120 min relative to administration of glucose. Hind-leg muscle was sampled via puncture biopsy at day −10 and +10 relative to parturition and was used to measure protein expression of the insulin-sensitive transporter SLC2A4. In the prepartum period, increased MET supply was associated with greater plasma glucose baseline values, maximum concentration of glucose after infusion, and reduced time required for plasma glucose concentration to return to baseline values and plasma glucose area under the curve (AUC). A similar response was observed for the insulin after glucose infusion; MET led to greater plasma insulin baseline values, maximum concentration of insulin after infusion, and plasma insulin AUC while the time required for plasma insulin concentration to return to baseline values was not affected by MET. Protein expression of SLC2A4 was greater in the MET-supplemented cows during the prepartum period. During the postpartum period, MET-supply increased plasma glucose AUC and none of the other parameters measured were affected by treatments. Overall, the data indicate differences in glucose utilization during the prepartum and postpartum period in response to methionine supply.