Study objectives were to determine whether electric heat blanket (EHB)-induced hyperthermia affects body temperature indices and production parameters similar to those generated in climate-controlled chambers. Multiparous Holstein cows (27) were subjected to 2 experimental periods (P): During P1 (4 d), cows were in thermoneutral conditions with ad libitum feed intake. During P2 (4 d), cows were assigned to 1 of 2 treatments: (1) thermoneutral conditions and pair-fed (PF; n = 8), (2) heat stress induced artificially by an EHB (Thermotex Therapy Systems Ltd. Calgary, Canada), with ad libitum feed intake (n = 19). Overall, the EHB markedly increased rectal temperature, vaginal temperature, skin temperature, respiration rate, and heart rate (1.3°C, 1.4°C, 1.1°C, 2-fold, and 15 bpm, respectively; P < 0.01) relative to PF cows. The EHB reduced DMI by 45% and by design PF cows had similar pattern of decreased DMI. Milk yield decreased in EHB and PF cows by 22.4% (9.8 kg) and 10.4% (4.2 kg), respectively (P = 0.03), indicating that reduced DMI accounted for only ~50% of decreased milk yield. Relative to PF cows, MUN increased (20.4%; P < 0.01) in EHB cows; however, no other differences were observed in milk composition between treatments. The EHB decreased blood total CO₂, partial CO₂, HCO₃, and base excess levels (15, 13, 15, and 78%, respectively; P < 0.01) when compared with PF group. The EHB increased hematocrit and hemoglobin (both indicators of dehydration) by (9%; P < 0.01) compared with PF cows. A treatment by day interaction was detected for plasma NEFA levels; as they progressively increased in PF controls but remained unchanged in EHB cows (P < 0.01). BUN and BHBA increased in EHB cows (31 and 83%, respectively; P < 0.01) relative to PF controls. No differences in circulating glucose were observed; however, insulin levels tended to increase (74%; P = 0.07) in the EHB cows compared with PF controls. In summary, employing the EHB increased body temperature indices, reduced productivity, and altered metabolism similar to climate controlled chamber studies.