Lactobacillus delbrueckii ssp. bulgaricus has been widely used for decades in the production of fermented products, especially yogurt and some cheeses. Several studies have linked the consumption of this species of bacteria to a reduced risk of colorectal cancer, the common cold, and inflammatory bowel disease. Consequently, due to the high demand for yogurt products in the United States, there is a need to isolate new strains of Lactobacillus bulgaricus to meet the increased yogurt production. In this study, we developed and evaluated Modified Reinforced Clostridial Medium (mRCM) for the differential enumeration of L. delbrueckii ssp. bulgaricus. Our results showed that supplementing Reinforced Clostridial Medium (RCM) with 0.025% CaCl₂, 0.01% uracil and 0.2% Tween 80 (mRCM) significantly (P < 0.05) enhanced the growth rate of L. bulgaricus RR and ATCC 11842 strains as measured by the optical densities of these strains after 12 h incubation at 42°C. The bacterial populations (plate count) of the RR and ATCC 11842 strains were 0.76 and 0.77 log cfu/g higher in mRCM than in de Man Rogosa and Sharpe (MRS) and RCM media, respectively. Conversely, other bacterial species (Bifidobacterium, L. rhamnosus, and L. reuteri) were significantly inhibited in the mRCM medium. The addition of aniline-blue dye to mRCM (mRCM-BLUE) improved the selectivity of L. bulgaricus in mixed lactic bacterial cultures compared with MRS and lactic agar (LA) with regard to colony appearance and morphology. mRCM-BLUE performed better than conventional media in culturing, enumerating, and differentiating L. bulgaricus. Thus, mRCM-BLUE, could be used as a selective medium to enhance the growth and differentiation of L. bulgaricus to meet the increasing demand for this beneficial species of bacteria.