Heat stress is a challenge for the US dairy industry, but few topical application strategies have been attempted to mitigate it. Sixty mid-lactation Holstein cows were used in a randomized block design experiment to evaluate impacts of a spray-on product on body temperature over 42 d. Cows were blocked by milk yield and coat color, and randomly assigned to treatments that differed in spray-on product quantity (200 or 300 mL) and application frequency (once on d 1, or twice, on d 1 and 24). The placebo treatment (300 mL water) was applied twice, providing a total of 5 treatments. Treatments were applied as an aerosol spray to both sides of the animal with a low volume, low pressure spray gun. Vaginal temperature (VT) was recorded every 5 min for 4 d beginning on d 9 and 30 using the ibutton/blank CIDR method. Infrared thermographic images (ITI) were taken from right and left side of rib cage of the cows 3 × /wk in the evening during wk 2, 4, and 6. ITI of the eye and base of udder were also collected during wk 2 and 6. Statistical models included the fixed effect of treatment and the random effect of block, with repeated measures over time. Pearson correlation coefficients were calculated to compare paired VT and maximum and mean temperatures from ITI. THI exceeded 72 for 14 h/d during the study, suggesting that heat stress likely affected cows. During d 9–13 after initial treatment, spray-on product treatments increased VT relative to control (38.72, 28.86, and 38.86 ± 0.06°C for 0, 200, and 300 mL, respectively; P < 0.01), but no treatment differences were observed on VT on d 30 - 34 (6–10 d after the second treatment). Treatments had no effect on mean ITI temperature of the eye, the base of the udder, or over the ribs. Eyeball and udder ITI maximum temperature were positively correlated (P < 0.01; R = 0.39 and 0.29, respectively), whereas the mean ITI of udder and left side of rib cage were negatively correlated (P ≤ 0.046, R = −0.36 and −0.19) with VT. We observed no evidence that the spray-on product reduces body temperature of cows. Maximum temperatures from ITI of eye had a moderately positive relationship with VT.