The objective of this study was to evaluate the effect of frequency of diet delivery to dairy cows on CH₄ emissions from stored manure. For this purpose, 12 lactating cows were used in a randomized block design and offered a TMR (56:44, forage:concentrate ratio) once/d (0930 h), twice/d (0930 and 2130 h), or 4 times/d (0930, 1300, 1630, and 2130 h). Manure (feces and urine) was collected from each cow on 2 consecutive days, mixed (1:1) with an inoculum from a bioreactor and stored (20°C) under anaerobic conditions in glass bottles (4 replicates/cow) for 17 weeks. Quantity of gas produced was measured 4 times/week and samples taken to determine gas composition. Data were analyzed using the MIXED procedure of SAS and significance was declared at P ≤ 0.05 using the Tukey test for multiple comparisons. Frequency of diet delivery did not affect the amount of manure excreted, which averaged 76 kg/d and 6.9 kg of volatile solids (VS)/d. Methane emission was unaffected (P = 0.18) by frequency of diet delivery (155, 164 and 125 L CH₄/kg VS; SEM = 15.9, for cows offered the diet once/d, twice/d, and 4 times/d; respectively). Volatile solids loss over the storage period averaged 27% and was not affected by frequency of diet delivery. In conclusion, under the conditions of this experiment, frequency of diet delivery to dairy cows had no effect on CH₄ emissions from stored manure.