The objective of this study was to examine the effect of frequency of diet delivery to dairy cows on enteric CH₄ emissions. Twelve lactating multiparous Holstein cows (DIM = 85 ± 23.7; milk yield = 50 ± 3.7 kg) were used in a replicated 3 × 3 Latin square design (35-d period; 14-d adaptation). Cows were offered (ad libitum, 5% orts; on an as-fed basis) a TMR (56:44, forage:concentrate ratio) once/d (0930 h), twice/d (0930 and 2130 h), or 4 times/d (0930, 1300, 1630, and 2130 h). Production of CH₄ was determined (5 consecutive days) using respiration chambers, while DMI, milk performance, and nutrient apparent-total-tract digestibility were determined over 7 consecutive days. Data were analyzed using the MIXED procedure of SAS and significance was declared at P ≤ 0.05 using the Tukey test for multiple comparisons. Frequency of diet delivery had no effect on DMI (26 kg/d), ECM yield (46 kg/d), and DM apparent-total-tract digestibility (71%). Daily enteric CH₄ emission averaged 534 g/d and was not affected by diet delivery frequency. However, CH₄ emission expressed on DMI basis or as a proportion of gross energy intake (GEI) was lower (P = 0.01) when diet was offered once/d (19.3 g/kg, 5.75%; respectively) compared with twice/d (20.3 g/kg, 6.06%; respectively) and 4 times/d (20.4 g/kg, 6.06%; respectively). Methane emission expressed on ECM yield basis averaged 11.7 g/kg and was not affected by diet delivery frequency. Results of this study show that increasing frequency of diet delivery increases enteric CH₄ losses (g/kg DMI; % GEI) without affecting DMI and milk production.