Acinetobacter baumannii is a major worldwide pathogen involved in healthcare-associated infections and is one of the most common causes of ventilator-associated pneumonia in the United States. There is growing concern with its ability to acquire resistance to multiple drugs and carbapenem-resistant A. baumannii (CRAB) are a focus since second-line treatments for A. baumannii infections are less defined in their efficacy and toxicity. We isolated various lactic acid bacteria (LAB) from Egyptian yogurt that were then tested for their ability to produce bacteriocins that would inhibit growth of CRAB. Tracheal aspirate isolates were collected from patients at Egyptian hospitals and 85 isolates were recognized as A. baumannii by PCR detection of bla_OXA-51. Eighty-two isolates had multiple drug resistance based on antimicrobial susceptibility testing. These CRAB isolates were tested for known drug-resistance genes, and 38% tested positive for bla_OXA-24 while only one isolate carried bla_OXA-58 indicating other genes may also be involved in providing drug-resistance. Cell-free extracts were prepared from the 25 LAB isolates and tested for inhibition of all 85 CRAB isolates using an agar diffusion bioassay. Four LAB isolates identified as Lactobacillus casei, Lactobacillus plantarum, Bifidobacterium bifidum and Streptococcus thermophilus had the most inhibitory effect, and inhibited growth of 61, 54, 71 and 60 of the CRAB isolates, respectively. The B. bifidum supernatant (BbV1) was purified using ion-exchange chromatography after which inhibition of CRAB isolates reached 97%. The BbV1 active agent was heat stable and based upon amino acid content it was a bacteriocin-like compound. Our finding demonstrated that B. bifidum BbV1 isolated from Egyptian yogurt produces an antimicrobial compound that is inhibitory against Acinetobacter infection in vitro. Further studies will be aimed at in vivo use of a probiotic yogurt containing B. bifidum BbV1 in animal and human clinical trials to determine its application in the treatment of CRAB infections.