Temporary cessation of milking for 3 d following intramammary infusion of antibiotics was established as a new treatment method for mastitis in Japan. Innate immunity shows quick response after pathogen invasion and has broad spectrum of antimicrobial activity. Innate immune components (antimicrobial peptides) are synthesized in the alveolar epithelial cells and leukocytes and are secreted into milk. We hypothesized that innate immunity has an important role on the mammary gland during treatment with cessation of milking. Therefore, we investigated the concentration of innate immune components during and around the use of this treatment in goat milk. Ten Tokara goats in the mid lactating stage were used. Milking of both udders was stopped temporarily for 3 d followed by the resumption of milking once a day. Milk was collected from 3 d before milking cessation until 7 d after resumption of milking. After milk yield was measured, somatic cells were counted. Concentration of lactoferrin and cathelicidin-7 (innate immune components) and lactoperoxidase activity in defatted milk were measured. The Kolmogorov-Smirnov test was used to check the normal distribution of milk yield, lactoferrin, cathelicidin-7 and LPO data. A non-parametric Kruskal-Wallis test followed by Steel-Dwass test was used to compare the data among different days. One-way ANOVA followed by Tukey multiplex analysis was used to compare SCC among different days. Probability at <0.05 was considered significant. Milk yield was decreased significantly on d 2 and 3 after the end of milking cessation compared with that before milking cessation (P < 0.05). Conversely, there was significantly higher SCC on d 2 after the end of milk cessation than that before milk cessation (P < 0.05). Lactoferrin and cathelicidin-7 concentrations were increased temporarily at d 2 and 3 after the end of milking cessation compared with that before cessation (P < 0.05). These results suggested that 3-d cessation of milking induces temporal augmentation of lactoferrin and cathelicidin-7 that is contribute in the treatment of mastitis.