Abstract #234
Section: Physiology and Endocrinology
Session: Physiology & Endocrinology II
Format: Oral
Day/Time: Monday 2:15 PM–2:30 PM
Location: 326
Session: Physiology & Endocrinology II
Format: Oral
Day/Time: Monday 2:15 PM–2:30 PM
Location: 326
# 234
Association between bone and energy metabolism in calcidiol treated dairy cows.
R. M. Rodney1,2, N. P. Martinez3, P. Celi4,5, J. E. P. Santos3, D. R. Fraser2, I. J. Lean*1,2, 1Scibus, Camden, NSW, Australia, 2School of Life and Environmental Sciences, Faculty of Veterinary Science, University of Sydney, Camden, NSW, Australia, 3Separtment of Animal Sciences, University of Florida, Gainesville, FL, 4DSM Nutritional Products, Animal Nutrition and Health, Columbia, MD, 5Faculty of Veterianry and Agricultural Sciences, The University of Melbourne, Parkville, VIC, Australia.
Key Words: IGF-1, osteocalcin, vitamin D
Association between bone and energy metabolism in calcidiol treated dairy cows.
R. M. Rodney1,2, N. P. Martinez3, P. Celi4,5, J. E. P. Santos3, D. R. Fraser2, I. J. Lean*1,2, 1Scibus, Camden, NSW, Australia, 2School of Life and Environmental Sciences, Faculty of Veterinary Science, University of Sydney, Camden, NSW, Australia, 3Separtment of Animal Sciences, University of Florida, Gainesville, FL, 4DSM Nutritional Products, Animal Nutrition and Health, Columbia, MD, 5Faculty of Veterianry and Agricultural Sciences, The University of Melbourne, Parkville, VIC, Australia.
There are beneficial effects of vitamin D and DCAD interventions in the pre-calving period on production, health and reproduction of cattle. How interventions during this time can have extended effects in the following lactation is not yet clear. Time series analysis was used to examine responses between metabolites in peri-parturient cows to evaluate roles of bone metabolism in the adaption to lactation. Holstein cows (n = 32) were blocked by parity and milk yield and randomly allocated to diets containing either 25-OH-D3 or vitamin D3 (3 mg/11 kg of DMI) and positive (+130 mEq/kg) or negative (−130 mEq/kg) DCAD from 255 d gestation to parturition. Blood was sampled every 3 d pre- and postpartum (14 samples/cow). Concentrations of 25-OH-D3, vitamin D3, osteocalcin (OC), crosslaps, IGF-1, glucose and insulin were determined. Feeding 25-OH-D3, compared with vitamin D3, increased blood concentrations of 25-OH-D3 pre- (264.2 vs 61.3 ± 8.0 ng/mL) and postpartum (170.8 vs 51.3 ± 6.2 ng/mL) and decreased concentrations of vitamin D3 (1.2 vs 14.5 ± 0.6 ng/mL pre- and 1.9 vs 3.2 ± 0.6 ng/mL postpartum for 25-OH-D3 and vitamin D3 respectively). Pre-partum, cows fed the negative DCAD diets had decreased blood concentrations of vitamin D3 and glucose. Nulliparous cows had increased concentrations of OC, crosslaps, IGF-1, glucose and insulin when compared with multiparous cows. Data for each cow were used to produce approximately stationary series and time series cross correlations between variables at 3d lags calculated for use in a meta-analytical evaluation of associations between metabolites over time. Associations indentified included those between 25-OH-D3 and IGF-1 (ES 0.2 to 0.5 at 3 d before, and ES −0.1 to −0.4 at −9 d before) and between vitamin D3 and IGF-1 (positive on the same d and 3 before, and negative at 6 and 9 d before and 6 d after). Associations between IGF-1, uOC, cOC, crosslaps, and glucose were also present. A feedback loop between bone and energy metabolism was identified as indicated by alternative positive and negative effect size estimates at different lags. The positive association between OC and IGF-1 may indicate an effect that could influence future production.
Key Words: IGF-1, osteocalcin, vitamin D
