Galectins (Gal) are a family of proteins that bind to β-galactoside sugars found on parts of other proteins, either on the cells’ surface or in the extracellular matrix. There are 15 galectin protein subtypes that all share the characteristic of carbohydrate recognition. Galectins are known to have an impact on immuno-modulation and are involved in uterine immunoregulation during pregnancy. The aim of this study was to evaluate the expression of Galectins in cow blood and identify their modulation during their periparturient period. We hypothesized that fold changes in galectin expression is dependent on the periparturient period (close-up and c+7) and also the health of the animal. Fold change ≥2 is considered significant. With the use of Primer-Blast from NCBI, galectin 1, 2, 3, 4, 7,8, 9,12 (forward and reverse) were sequenced for use in this project. Blood was taken 2 weeks close to calving (close up), and 7 d after calving(c+7) at Michigan State University dairy farm and shipped in Paxgene tubes for analysis (n = 20). Total RNA was isolated, reverse-transcribed to cDNA, and then used in real-time PCR experiments with the above primers and β-actin as the control. Fold change in transcript abundance was calculated using the Livak method. Where ΔΔCt = (Target genes_c+7 - βactin_c+7) - ΔCt (Target genes _closeup - βactin _close-up). Fold change = 2^(-ΔΔCt). In non-pregnant cows, all 8 galectins tested were expressed in bovine blood. In periparturient cows, only 1, 2, 3 and 4 were expressed. Galectins 1, and 3 were expressed in both close-up and C+7 samples. Galectin 4 was expressed before parturition. Galectin 2 was expressed after parturition. All genes tested were expressed in cow blood at varying levels. Galectin 1 was upregulated after calving (Fold change = 2.4). Further studies are needed to determine the role of galectins and factors that affect their expression in blood during the periparturient period.