Clinical assessment during hoof trimming implies restrictive methods and many hours of keeping cows waiting for their turn. The intention of this study was to characterize temperature emission in punctual areas of the hoof by capturing images with an infrared thermography camera (IRT) comparing them to other hoof conditions. The trial took place on a commercial dairy in Central México. Punctual temperature emission was used by tracking 14 specific areas in the sole. Mapping was related to the external/internal middle axis: Sp1/Sp9 (white line axial wall); Sp2/Sp10 (white line); Sp3/Sp11 (abaxial wall); Sp4/Sp12 (caudal claw); Sp5/Sp13 (cranial claw); Sp6/Sp14 (heel bulb); Sp7 (interdigital hind claw); Sp8 (interdigital medial claw). The study included a convenience sampling of 37 milking cows (<150 DIM), in an ambient temperature of 23°C. Cows were restrained in the hydraulic chute for hoof trimming, clinical analysis and IRT image capturing. Hoof analysis was characterized as sole ulcers, digital dermatitis, white line disease and normal cases. Two multiple statistical comparison tests (Hsu’s MCB test/Wilcoxon pairwise contrast) showed differences in temperature between heel bulb with ulceration and the other areas (JMP 11.1). The hind-left hoof showed an average temperature of 29.5°C in area Sp14 in cows with sole ulcer (P = 0.0003). For the hind-right hoof, sole ulcer cases were different from the normal in 3 instances: area Sp3 with a temperature of 22.9°C (P = 0.03); and areas Sp6 and 14 with average temperatures of 21.2 and 18.8°C respectively (P = 0.05; P = 0.0027). The most common cases overall were slight dermatitis in both hind limbs (49%); and sole ulcers in the left hoof (7.3%). Areas Sp6 and 14 are easily reached without causing distress in cows during milking, currently under investigation. The diagnostic approach is a non-invasive system that identifies elevated emission of temperature of the heel bulb related to ulcers when taking an IRT image.