Abstract #M36
Section: ADSA Production PhD Poster Competition (Graduate)
Session: ADSA Graduate Student (PhD) Production Poster Competition
Format: Poster
Day/Time: Monday 7:30 AM–9:30 AM
Location: Exhibit Hall B
Session: ADSA Graduate Student (PhD) Production Poster Competition
Format: Poster
Day/Time: Monday 7:30 AM–9:30 AM
Location: Exhibit Hall B
# M36
Effects of replacing soybean meal with canola meals varying in rumen undegraded protein on ruminal fermentation in vitro.
H. F. Monteiro*1, E. M. Paula1, J. L. P. Daniel2, P. D. B. Benedetti3, R. Bittner1, L. G. Silva1, T. Shenkoru1, A. P. Faciola1, 1University of Nevada, Reno, Reno, NV, 2State University of Maringá, Maringá, PR, Brazil, 3Federal University of Viçosa, Viçosa, MG, Brazil.
Key Words: degradability kinetics, metabolizable energy, methane
Effects of replacing soybean meal with canola meals varying in rumen undegraded protein on ruminal fermentation in vitro.
H. F. Monteiro*1, E. M. Paula1, J. L. P. Daniel2, P. D. B. Benedetti3, R. Bittner1, L. G. Silva1, T. Shenkoru1, A. P. Faciola1, 1University of Nevada, Reno, Reno, NV, 2State University of Maringá, Maringá, PR, Brazil, 3Federal University of Viçosa, Viçosa, MG, Brazil.
This study aimed to evaluate the effects of replacing soybean meal (SBM) with canola meals (CM) differing in rumen-undegraded protein (RUP; % of CP) content (38% RUP, LCM or 50% RUP, HCM) on in vitro ruminal fermentation, including total gas and CH4 production. Two in vitro experiments were conducted. Both experiments had 3 48 h incubations, totaling 18 observations per treatment. Experiment 1 tested 3 protein supplements as the sole ingredient (SBM, LCM, and HCM). Experiment 2 tested 3 TMR containing the protein supplements from experiment 1. Measurements were: pH, gas production (GP), degradability kinetics, metabolizable energy (ME), in vitro true organic matter digestibility (iv-tOMd), CH4 and volatile fatty acids (VFA) production. Mixed linear models were used to analyze the data. Degradability kinetics were fitted using nonlinear regression. Means were compared by orthogonal contrasts: SBM vs. (LCM + HCM) and LCM vs. HCM. Partial data are presented in Table 1. The SBM ingredient had greater iv-tOMd, VFA production, ME, total GP48 and CH4 (mM) when compared with both CM. When comparing both CM ingredients, HCM had shorter lag phase and lower branched-chain VFA production. However, ingredients did not differ in CH4 (g/kg dOM). In experiment 2, SBM diet had a trend to increase total CH4 production (mM), but diets did not differ in CH4 (g/kg dOM). The SBM diet also had a trend to increase valerate and iso-valerate concentration which may indicate more proline and leucine deamination. When comparing both CM diets, HCM increased final pH and had a trend to lower ME, while decreased total GP48.
Table 1.
| Item | Treatment | SEM | P-value | |||
| SBM | LCM | HCM | SBM vs. | LCM vs. | ||
| Experiment 1 (sole ingredients) | ||||||
| Total GP48, mL/g of DM | 284 | 240 | 233 | 10.30 | <0.01 | 0.49 |
| iv-tOMd, % | 98.3 | 80.1 | 81.4 | 1.07 | <0.01 | 0.31 |
| ME, MJ/kg of DM | 15.1 | 12.9 | 12.8 | 0.30 | <0.01 | 0.65 |
| CH4, mM | 15.7 | 12.6 | 11.7 | 0.56 | <0.01 | 0.19 |
| CH4, g/kg of dOM | 41.6 | 40.9 | 38.2 | 1.88 | 0.30 | 0.18 |
| Experiment 2 (TMR) | ||||||
| Total GP48, mL/g of DM | 309 | 312 | 276 | 10.40 | <0.01 | <0.01 |
| iv-tOMd, % | 75.0 | 71.5 | 69.9 | 1.90 | <0.01 | 0.11 |
| ME, MJ/kg of DM | 11.1 | 10.4 | 10.1 | 0.33 | <0.01 | 0.07 |
| CH4, mM | 14.2 | 12.7 | 12.6 | 0.98 | 0.07 | 0.93 |
| CH4, g/kg of dOM | 40.8 | 41.5 | 40.1 | 5.26 | 0.99 | 0.63 |
Key Words: degradability kinetics, metabolizable energy, methane
