Abstract #40
Section: ADSA Southern Section Oral Competition (Graduate)
Session: ADSA Southern Section Graduate Student Oral Competition
Format: Oral
Day/Time: Monday 10:30 AM–10:45 AM
Location: 318
Session: ADSA Southern Section Graduate Student Oral Competition
Format: Oral
Day/Time: Monday 10:30 AM–10:45 AM
Location: 318
# 40
Efficacy of calcium montmorillonite clay at reducing aflatoxin transfer in lactating Holsteins fed a known concentration of aflatoxin.
S. C. Allen*1, Z. A. Mason1, B. J. Rude1, R. H. Bailey1, C. Maki2, T. Phillips2, S. H. Ward3, 1Mississippi State University, Mississippi State, MS, 2Texas A&M University, College Station, TX, 3North Carolina State University, Raleigh, NC.
Key Words: aflatoxin
Efficacy of calcium montmorillonite clay at reducing aflatoxin transfer in lactating Holsteins fed a known concentration of aflatoxin.
S. C. Allen*1, Z. A. Mason1, B. J. Rude1, R. H. Bailey1, C. Maki2, T. Phillips2, S. H. Ward3, 1Mississippi State University, Mississippi State, MS, 2Texas A&M University, College Station, TX, 3North Carolina State University, Raleigh, NC.
Fifteen lactating Holstein cows were used in a 5 × 5 LS design to analyze the efficacy of NovaSil Plus (NSP), a calcium montmorillonite clay, at preventing aflatoxin (AF) contamination in milk. Cows were housed in a free-stall barn with sand bedding and were individually fed and milked twice daily. The study consisted of 5 10-d periods. Treatment was given d 1 to 5, and d 6 to 10 were considered a washout period to prevent carryover. Cows were predicted to consume 25 kg of dry matter (DM)/d, AF was fed at 50 ppb. NovaSil Plus was fed at 3 concentrations: 0.125, 0.25, and 0.5% predicted DM intake (DMI). Cows were randomly assigned to 1 of 5 dietary treatments: (1) control (CON), total mixed ration (TMR) with no AF or NSP; (2) AF control (AFC), TMR with 50 ppb AF; (3) NSP control (NSPC), TMR with 0.5% DMI NSP; (4) low-dose clay with AF (NSP-0.125%), TMR with 50 ppb AF and 0.125% DMI NSP; and (5) high-dose clay with AF (NSP-0.25%), TMR with 50 ppb AF and 0.25% DMI NSP. All treatments were top dressed. Data were analyzed using the MIXED procedure of SAS® (Cary, NC) where period and treatment were the main effects. Significance was declared when P ≤ 0.05. Milk yield and feed efficiency were similar among treatments, and milk composition was similar with the exception of fat (P < 0.01) and protein (P < 0.01) content. Fat content averaged 4.22, 4.50, 4.38, 4.75, and 4.61% for CON, NSPC, AFC, NSP-0.125%, and NSP-0.25% respectively. Protein content averaged 2.93, 2.96, 2.98, 2.92, and 3.02% for CON, NPSC, AFC, NSP-0.125%, and NSP-0.25% respectively. A dose dependent response was observed with the inclusion of NSP (P < 0.01). Aflatoxin M1 averaged 0.09, 0.03, 0.75, 0.58, and 0.65 ppb. Aflatoxin M1 was reduced by 13.3% (0.65 ± 0.05 μg/L) and 22.7% (0.58 ± 0.05 μg/L) in cows fed NSP-0.125% and NSP-0.25% diets, respectively, when compared with cows fed AFC diets. Cows administered NSP secreted less AFM1 than cows consuming AFC diets. Aflatoxin M1 secretion (P < 0.01) averaged 2.99, 0.98, 27.33, 21.18, and 23.60 μg/d for CON, NSPC, ASPC, NSP-0.125, and NSP-0.25 diets respectively. This study showed that feeding NSP at smaller doses will reduce transfer of AFM1 into milk and provide a lower cost solution to dairy producers.
Key Words: aflatoxin
