Abstract #109
Section: Ruminant Nutrition
Session: Ruminant Nutrition I
Format: Oral
Day/Time: Monday 10:30 AM–10:45 AM
Location: 321
Session: Ruminant Nutrition I
Format: Oral
Day/Time: Monday 10:30 AM–10:45 AM
Location: 321
# 109
Effect of sequestering agents based on a Saccharomyces cerevisiae fermentation product and clay on the performance of lactating dairy cows challenged with dietary aflatoxin B1.
Y. Jiang*1, D. H. Kim1, I. M. Ogunade1, X. Li2, A. A. Pech-Chevantes1, A. S. Oliveira3, K. G. Arriola1, A. Mayer-Camocho1, J. P. Driver1, C. R. Staples1, D. Vyas1, A. T. Adesogan1, 1Department of Animal Sciences, University of Florida, Gainesville, FL, 2Department of Animal Sciences, China Agricultural University, Beijing, China, 3Institute of Agriculture and Environmental Sciences, Federal University of Mato Grosso, Sinop, MT, Brazil.
Key Words: aflatoxin, milk, clay
Effect of sequestering agents based on a Saccharomyces cerevisiae fermentation product and clay on the performance of lactating dairy cows challenged with dietary aflatoxin B1.
Y. Jiang*1, D. H. Kim1, I. M. Ogunade1, X. Li2, A. A. Pech-Chevantes1, A. S. Oliveira3, K. G. Arriola1, A. Mayer-Camocho1, J. P. Driver1, C. R. Staples1, D. Vyas1, A. T. Adesogan1, 1Department of Animal Sciences, University of Florida, Gainesville, FL, 2Department of Animal Sciences, China Agricultural University, Beijing, China, 3Institute of Agriculture and Environmental Sciences, Federal University of Mato Grosso, Sinop, MT, Brazil.
The objective was to examine the effect of supplementing bentonite clay with or without a Saccharomyces cerevisiae fermentation product (SCFP; 19 g Diamond V NutriTek + 16 g MetaShield) on the performance and liver function enzymes of dairy cows challenged with aflatoxin B1 (AFB1). Twenty-four Holstein cows (64 ± 11 DIM) were stratified by parity and milk production and randomly assigned to 1 of 4 treatment sequences. The experiment had a balanced 4 × 4 Latin square design with 6 replicate squares, 4 33-d periods and a 5-d washout interval between periods. Cows were fed a TMR containing 36.1% corn silage, 8.3% alfalfa hay and 55.6% concentrate (DM basis). Treatments were: 1) Control (no additives); 2) Toxin (1,725 µg/head/d of AFB1; T); 3) T + Clay (200 g/hd/d; top-dressed; CL); and 4) CL + SCFP (35 g/hd/d; top-dressed; CL+SCFP). Cows were adapted to diets from d 1 to 25 (pre-dosing period) and AFB1 was orally dosed from d 26 to 30 (dosing period) and withdrawn from d 31 to 33 (withdrawal period). Milk samples were collected twice daily from d 26 to 33 and plasma was sampled for liver enzymes on d 25 and 30 before the morning feeding. Data were analyzed with the GLIMMIX procedure of SAS. Transfer of ingested AFB1 into milk aflatoxin M1 (AFM1) was greater in T than CL or CL+SCFP (1.65 vs. 1.01 and 0.94%, respectively, P < 0.0001) on d 26 to 30. The CL and CL+SCFP treatments reduced milk AFM1 concentration compared with T (0.45 and 0.40 vs. 0.75 µg/kg, respectively; P < 0.0001) and unlike T, they resulted in lower AFM1 concentrations than the FDA action level (0.5 µg/kg). Milk yield tended to be greater during the dosing period in cows fed CL+SCFP instead of T (39.7 vs. 37.7 kg/d, P = 0.08). Plasma glutamic oxaloacetic transaminase concentration, indicative of aflatoxicosis and liver damage, was numerically increased by T (87.9 vs. 95.2 U/L, P = 0.11) but reduced by CL compare with T (85.9 vs. 95.2 U/L, P = 0.03), adding CL+SCFP result in similar level as Control (87.9 vs. 88.7 U/L, P = 0.99). Adding CL or CL+SCFP to the diet reduced AFB1 transfer to milk and AFM1 excretion but only CL+SCFP prevented the decrease in milk yield caused by ingestion of the toxin.
Key Words: aflatoxin, milk, clay
