Colostrum composition is influenced by many maternal and environmental conditions; however, it is unknown what influences the calf has on colostrum components. The objective of this study was to determine the relationship between postpartum calf management techniques on colostrum components. Prior to birth, Angus (Ang; n = 33) and Charolais (Char; n = 35) calves were allotted to 1 of 5 treatment groups: calves naturally reared and suckled from their dam (AngCON, n = 21 or CharCON, n = 25), calves born via C-section (AngC-Sect, n = 3 or CharC-Sect, n = 10), or calves administered 5 g of a commercial probiotic (AngPROB, n = 9; Probios) shortly after birth. There was no probiotic treatment for Charolais calves. Colostrum was collected at 24 h post-calving, placed on ice, and stored at −4°C until analyses. Samples were analyzed for percentage fat, percentage true protein, percentage solids nonfat, lactose, somatic cell count, acetone, β-hydroxybutyrate, and urea. All components were non-normally distributed; therefore, the transreg procedure in SAS was used to determine appropriate transformations. An ANOVA was used to determine the effect of treatment with birth weight and sex included as fixed effects for each breed separately. An ad hoc analysis with a Tukey adjustment was used to account for multiple comparisons. In Angus, colostrum true protein, solids nonfat, and urea levels were increased in AngPROB compared with AngCON; whereas, lactose levels decreased. Somatic cell count increased for AngPROB compared with both AngCON and AngC-Sect. In Charolais, urea was increased in CharC-Sect (34.39 ± 3.62 dL/mg) compared with CharCON (17.60 ± 1.96 dL/mg). Additionally, Colostrum true protein and solids nonfat tended to increase in CharC-Sect compared with CharCON. Interestingly, similar patterns were observed for colostrum components between Angus and Charolais for mode of delivery (i.e., natural birth versus C-Section). It is possible that how a calf is managed during and(or) shortly after birth may impact colostrum composition. However, more research is needed to elucidate these relationships.