Abstract #T148
Section: Physiology and Endocrinology
Session: Physiology & Endocrinolog II
Format: Poster
Day/Time: Tuesday 7:30 AM–9:30 AM
Location: Exhibit Hall B
Session: Physiology & Endocrinolog II
Format: Poster
Day/Time: Tuesday 7:30 AM–9:30 AM
Location: Exhibit Hall B
# T148
Fatty acid composition of red blood cell membranes of dairy cows fed a diet with low n-3 fatty acid content and effects of fatty acid supplementation.
D. Revskij1, S. Haubold1, C. Kröger-Koch1, H. Kienberger2, M. Rychlik3, A. Tuchscherer1, A. Tröscher4, H. J. Schuberth5, H. M. Hammon1, M. Mielenz*1, 1Leibniz Institute for Farm Animal Biology (FBN), Dummerstorf, Germany, 2Bavarian Biomolecular Mass Spectrometry Center, Technical University of Munich, Freising, Germany, 3Analytical Food Chemistry, Technical University of Munich, Freising, Germany, 4BASF SE, Limburgerhof, Germany, 5Immunology Unit, University of Veterinary Medicine, Hannover, Germany.
Key Words: dairy cow, fatty acids, red blood cells
Fatty acid composition of red blood cell membranes of dairy cows fed a diet with low n-3 fatty acid content and effects of fatty acid supplementation.
D. Revskij1, S. Haubold1, C. Kröger-Koch1, H. Kienberger2, M. Rychlik3, A. Tuchscherer1, A. Tröscher4, H. J. Schuberth5, H. M. Hammon1, M. Mielenz*1, 1Leibniz Institute for Farm Animal Biology (FBN), Dummerstorf, Germany, 2Bavarian Biomolecular Mass Spectrometry Center, Technical University of Munich, Freising, Germany, 3Analytical Food Chemistry, Technical University of Munich, Freising, Germany, 4BASF SE, Limburgerhof, Germany, 5Immunology Unit, University of Veterinary Medicine, Hannover, Germany.
Incorporation of dietary n-3 fatty acids (FA) in plasma membranes influences membrane fluidity and function. Common diets for dairy cows often deliver lower amounts of n-3 FA compared with pasture-based systems. Objective was to evaluate effects of a diet low in n-3 FA (Trial 1) and with additional FA supplementation (Trial 2) on the FA composition of red blood cell (RBC) membranes. Cows were fed a corn silage based TMR for 24 weeks (n = 5) in Trial 1 and 12 weeks in Trial 2 (n = 4). In Trial 1 RBC were collected in week −1, 0, 1, 2, 8, 16, 24. In Trial 2 cows, fitted with abomasal infusion tubes, were arranged in a 4 × 4 Latin square model after the 12 weeks and supplemented with 3 successively rising lipid dosages for 2 weeks, respectively, followed by a 3 week wash out phase. Supplements were coconut oil, linseed-safflower oil mix, the latter delivering n-3 FA (EFA), LUTALIN (c9,t11 + t10,c12 CLA; Ludwigshafen, Germany) or both, EFA + CLA. Membranes were isolated at the end of each treatment and wash out period. Data were analyzed with the MIXED procedure of SAS using a repeated measurement ANOVA model with treatment as fixed effect and week in milk as covariate in Trial 2. LSMEANS were compared by Tukey test (±SEM). In Trial 1 relative content of total n-3 FA decreased (2.3 to 0.9 ± 0.3% (P < 0.001), eicosapentaenoic acid (EPA) 0.7 to 0.2 ± 0.1% (P < 0.001), a-linolenic acid 0.9 to 0.4 ± 0.1% (P < 0.001)), whereas the relative content of total n-6 FA increased (22.2 to 30.6 ± 1.7% (P < 0.01); linoleic acid (LA) 17.8 to 25 ± 1.4% (P < 0.01); arachidonic acid 3.2 to 3.9 ± 0.4% (P < 0.05)). In Trial 2 EFA + CLA treatment decreased the relative content of total n-6 FA and LA (37.3 to 31.2 ± 1.8% (P < 0.05); 28.6 to 24.1 ± 1.3% (P < 0.05)). Total amount of n-3 FA was not affected but EPA was increased by EFA and EFA + CLA treatment (1.1 to 2.3 ± 0.2% (P < 0.01); 1.2 to 2.1 ± 0.2% (P < 0.05)). In conclusion, an n-3 FA supplementation reduces the relative content of incorporated n-6 FA in plasma membranes, which might positively affect membrane function.
Key Words: dairy cow, fatty acids, red blood cells
