Glucagon-like peptide 1 (GLP-1) is involved in glucose homeostasis by stimulating insulin secretion while GLP-2 increases mucosal growth of the small intestine. The objective of this study was to evaluate effect of delayed colostrum feeding on plasma concentrations of GLP-1 and 2. Holstein bull calves (n = 27) were fed colostrum at 7.5% of BW within 1 h of birth (0H), 6 h after birth (6H) or 12 h after birth (12H; n = 9 for each treatment), and fed a milk replacer at 2.5% of BW at 12 h after the colostrum feeding and every 6 h thereafter. Blood samples were obtained before the colostrum feeding and every 3 h after the colostrum feeding for a 36-h period, and plasma concentrations of GLP-1, GLP-2 and glucose were measured. Data were analyzed by ANOVA to determine effects of treatment, time as repeated measures, and their interaction. Time by treatment interaction was observed for plasma GLP-1 concentrations (P = 0.04); GLP-1 concentrations increased after colostrum feeding (P < 0.01) regardless of treatment, but they tended to peak faster (P = 0.10) for 0H (12.7 ± 2.6 h; LSM ± SEM) than 6H (20.7 ± 2.6 h) or 12H (18.7 ± 2.6 h) calves, and its concentrations at 12 h after colostrum feeding were higher for 0H (3.37 ± 0.53 ng/mL) than 12H (2.11 ± 0.53 ng/mL). In addition, area under the curve (AUC) for GLP-1 concentrations was higher (P < 0.01) for 0H (6.65 ± 0.82 ng/mL × h) than 6H (3.12 ± 0.82 ng/mL × h) or 12H (2.23 ± 0.82 ng/mL × h). Similarly, plasma concentrations of GLP-2 and glucose increased after colostrum feeding (P < 0.01), and reached their peak concentrations at 15.4 ± 3.1 and 26.6 ± 1.9 h after the feeding, respectively. However, treatment did not affect plasma concentrations of GLP-2 and glucose, time to reach their peak concentrations, and their AUC for a 36-h period after colostrum feeding. In conclusion, delayed colostrum feeding may decrease plasma GLP-1 concentrations for a 36-h period after colostrum feeding without affecting plasma concentrations of GLP-2 and glucose.