Abstract #507

# 507
Triladyl improves post-thaw semen quality of Sahiwal bulls.
A. Sattar*1, S. Ali1, S. Firyal2, M. A. Khan1, A. Rehman1, M. U. Mehmood1, A. Rehman1, M. Z. Tahir1, 1Department of Theriogenology, University of Veterinary and Animal Sciences, Outfall Road, Lahore, Pakistan, 2Institute of Biochemistry and Biotechnology, University of Veterinary and Animal Sciences, Outfall Road, Lahore, Pakistan.

Semen cryopreservation is an important tool to improve the reproductive/ productive efficiency through artificial insemination (AI) in Sahiwal cattle (Bos indicus). The objective of present study was to compare the efficacy of commercially and traditionally used egg yolk based extenders (Triladyl vs. TCEY) on pre-freeze and post thaw semen quality of Sahiwal bull. For this purpose, semen from 5 Sahiwal bulls was collected using an artificial vagina (42?C) twice a week (replicates = 7). Ejaculates from each bull passing minimum criteria were selected for further processing. Then, semen samples of all bulls were pooled and 2 aliquots of pooled semen samples were made. Each aliquot was extended separately with specific extender, one with Triladyl and other with TCEY respectively. Final concentration of spermatozoa was kept 40 × 106 /mL. Before freezing, semen samples of both groups were evaluated for sperm motility parameters and kinematics by computer assisted semen analyzer (CASA). Diluted semen was cooled to 4?C, equilibrated for 4 h and filled in 0.5-mL French straws (20 × 106 sperm per straw). All semen straws were placed in automatic freezer (IMV-France) in liquid nitrogen vapors, then stored in liquid nitrogen. Post thaw motility (PTM), plasma membrane integrity (PMI), normal apical ridge (NAR).DNA integrity, live percentage, sperm abnormalities, mitochondrial activity and post thaw CASA motility parameters/ kinematics were studied. Results were compared statistically using independent t-test. Pre-freeze CASA sperm motility parameters (Progressive %, Rapid %, Medium %, Slow %) and kinematics VAP (um/s),VSL(um/s),LIN (%) were significantly (P < 0.05) better in Triladyl than TCEY while post thaw CASA sperm motility parameters (motile %, progressive %, rapid %, medium %, slow %, static %) and kinematics VAP (um/s),VSL (um/s) and STR (%) were also significantly (P < 0.05) better in Triladyl than TCEY extender. Post thaw semen quality parameters containing PTM, PMI, DNA integrity, live percentage, sperm abnormalities and mitochondrial activity were also significantly better (P < 0.05) in Triladyl as compared with TCEY. It may be concluded that commercially available extender Triladyl improves post thaw semen quality than TCEY extender in Sahiwal bulls.

Key Words: Triladyl, TCEY, Sahiwal bulls