Semen cryopreservation is crucial to introduce high genetics in buffalo herds through artificial insemination. Success of artificial insemination in Nili-Ravi buffalo (Bubalus bubalis) is limited due to poor freezing ability. The objective of this study was to compare the effect of commercial (Triladyl) and Tris-citric-egg-yolk (TCEY) semen extenders on post- thaw semen quality of Nili-Ravi buffaloes. Semen was collected using an artificial vagina (42°C) twice a week from 3 mature regular donor bulls of Nili-Ravi buffalo (7 replicates). After evaluation, semen was pooled and divided into 2 equal aliquots. These semen aliquots were extended separately in Triladyl and TCEY extenders, to a final concentration of 40 × 10⁶ sperms /mL. After filling in 0.5-mL French straws semen samples were frozen first in liquid nitrogen vapors using automatic freezer (IMV- France) and then stored in Liquid Nitrogen. Post-thaw motility (PTM), Plasma membrane integrity (PMI), Normal apical ridge (NAR), DNA integrity (DI), Live percentage (LP), Sperm abnormalities (SA) were studied using routine procedures. CASA (Pre-freeze and Post- Thaw) motility parameters and kinematics were also studied. Results were compared statistically using paired t-test. Post- thaw semen quality parameters were significantly better (P < 0.05) in Triladyl as compared with TCEY Extender. Pre freeze and post- thaw CASA motility parameters were also better in Triladyl semen extender. Pre-freeze CASA sperm motility kinematics VAP (µm/s), VSL (µm/s), LIN (%) and post- thaw CASA sperm motility kinematics VAP (µm/s), VSL (µm/s), and STR (%) were also found significantly (P < 0.05) better in Triladyl semen extender as compared with TCEY extender. It may be concluded that Triladyl semen extender may be a good choice for buffalo semen freezing as compared with TCEY extender.