Trace minerals and vitamins associated with antioxidant defense improve the immune response in dairy cattle during critical periods such as peripartum. Metritis represents a severe consequence of immune failure. The objective of the current study was to evaluate the effect of prepartum trace mineral and vitamin supplementation on the incidence of metritis in early lactation dairy cows. The trial was realized in a commercial dairy herd in Buenos Aires, Argentina. The herd was comprised of 574 primiparous and multiparous Holstein cows that were randomly divided in 2 groups: supplemented (n = 301) and non-supplemented (n = 273). Supplementation consisted of 3 parenteral applications of trace minerals and vitamins (Cu: 50 mg, Zn: 200 mg, Mn: 50 mg, Se: 25 mg, Vit. A: 315000 IU, and Vit. E: 250 IU - Adaptador, Biogénesis Bagó SA). Treatment was applied at drying off (60 d before calving), 21 d before calving, and at calving. The effect of treatment on the incidence of metritis was assessed by logistic regression analysis. The model included the fixed effects of mineral and vitamin supplementation (supplemented vs. non-supplemented), parity (1 vs. 2+) and their interaction. For the main and interaction effects, the α level of significance was set at P < 0.05 and P < 0.1, respectively. The incidence for metritis shows a treatment by parity interaction (P = 0.09). The risk of metritis decreased in supplemented multiparous cows, but not in primiparous cows compared with non-supplemented. Supplemented multiparous cows had a lower prevalence of metritis than non-supplemented (14 vs 26%, respectively). Conversely, the percentage of affected primiparous cows was similar for those receiving or not receiving the supplementation (32 vs. 30%, respectively). In conclusion, supplementation with a mix of antioxidant trace minerals and vitamins reduces the odds for metritis in multiparous cows.